Rafael Della Coletta

Ph.D. candidate at University of Minnesota

Project

Transposons in a maize population

Brief description

Transposable elements (TEs), or "jumping genes", are hard to study due to their repetitive nature and abundance in crop genomes. However, when using short-read sequencing data to characterize them in a population, things get even more complicated. In this study, we implemented a machine learning method to classify the presence and absence of TEs in a diversity panel with 500+ maize lines. By calculating linkage disequilibrium (LD) between TEs and single nucleotide polymorphisms (SNPs), I observed that nearly 20% of the TEs were not being tagged by an SNP. This result potentially represents information that has not been well captured in previous SNP-based marker-trait association studies. The step-by-step of the LD analysis can be found on this Github page.

Software

UNIX, TASSEL, Plink, R, bedtools, vcftools

Manuscript

Whole genome variation of transposable element insertions in a maize diversity panel (doi: 10.1093/g3journal/jkab238)

Authors

Qiu Y, O’Connor CH, Della Coletta R, et al.

Journal

G3: Genes, Genomes, Genetics

(impact factor: 3.154)

Year

2021